Oct 30, 2024
How to Increase the Fluorescence Intensity of Mitophagy Detection Kits?You can appropriately extend the incubation time of Mtphagy Dye or Lyso Dye.Example: Incubation time: 30 min → 60 minIf the stai...
Oct 20, 2024
When it comes to "vector construction," the first thing that might come to mind is the restriction enzyme method, which involves cutting with restriction endonucleases to produce sticky ends...
Oct 10, 2024
pBR322 is one of the earliest and most widely used cloning vectors in Escherichia coli. Created in 1977 at the University of California, San Francisco, in the laboratory of Herbert Boyer, it is named ...
Sep 30, 2024
Lentiviral vector construction is a key technology in modern biomedical research. This article provides a detailed explanation of the definition and characteristics of lentiviral vector construction, ...
Sep 23, 2024
Adeno-Associated Virus (AAV) belongs to the Parvoviridae family and is a non-enveloped, single-stranded linear DNA virus. Due to its advantages such as a wide host range, high safety, low immunogenici...
Sep 18, 2024
Introduction to Seamless Cloning TechnologySeamless cloning is a new, simple, rapid, and restriction enzyme site-independent technology. This technology depends on homologous sequence-based gene cloni...
Aug 28, 2024
Definition of AdenovirusAdenovirus is a spherical, non-enveloped virus with a diameter of approximately 70-90nm. Its capsid has a 20-sided icosahedral symmetry and is composed of 252 capsomers, includ...
Jul 31, 2024
Lentivirus is a type of retrovirus with a double-stranded RNA genome. Unlike typical retroviruses, lentiviruses have a broader host range and can infect both dividing and non-dividing cells.Recombinan...
Jul 22, 2024
Virus-based gene vectors are common tools in molecular biology that utilize viral carriers to deliver target genes to cultured cells or animals. Currently, commonly used viruses include lentivirus (LV...
May 30, 2024
Adenoviruses were discovered in 1953 in human studies of the common cold disease. Adenoviruses usually infect humans by infecting the eye, respiratory tract, or gastrointestinal epithelial cells, etc....
