Adenoviruses were discovered in 1953 in human studies of the common cold disease. Adenoviruses usually infect humans by infecting the eye, respiratory tract, or gastrointestinal epithelial cells, etc. In 1977, Dr. Frank Graham modified and recombined adenoviruses in cells, and since then, adenoviruses have been widely exploited as a means of gene delivery.
Adenovirus is a spherical, non-enveloped, particulate virus with a diameter of about 70 to 80 nm. Its genome is a 36-kb double-stranded linear DNA with inverted repeats of about 100 bp at each end, encoding more than 30 "regulatory proteins". The Fiber Knob on the surface of the adenovirus enters the cell through cellular endocytosis mediated by the Coxsackie adenovirus receptor (CAR) on the cell surface, and the adenovirus genome is then transferred to the nucleus, free from the chromosome.
Adenovirus packaging is the process of cloning the target fragment into an adenovirus expression vector, and the expression vector and packaging plasmid are recombinantly introduced into the adenovirus packaging cells for packaging. The adenovirus packaging system is divided into two main types according to the recombination method. One is the AdEasyTM Adenovirus Recombination System for recombination in bacteria (BJ5183). The other one is recombined in QBI-293A cells and is called AdMax Adenovirus Recombination System. This system is easy to operate, has high recombination efficiency, high yield of virus obtained, and high expression level of target gene.
Adenovirus packaging is an experimental technique in which the core principle is to embed the target genome into an adenoviral vector, and subsequently utilize packaging cells to transform this vector into intact adenoviral particles capable of infecting cells. The specific operation includes the steps of selecting an appropriate adenoviral vector, inserting the target genome, transfecting the packaging cells, adenoviral replication and packaging, collecting the adenovirus, and evaluating the packaging efficiency. This technology is widely used to produce adenoviruses for gene transfection, gene knockout, gene silencing, and other experiments.
Adenovirus packaging has a wide range of host cells, low pathogenicity, and high safety profile. It can effectively carry out proliferation with high titer and is suitable for gene therapy. The high infection efficiency can be expressed in 1-2 days, making it the best choice for studying gene expression in primary and suspension cells. It is used without integration into the genome and does not interfere with other host genes.
Hanbio's adenovirus packaging is effective in infecting host cells and meets the needs of many researchers, and Hanbio provides comprehensive support with professional services in the biomedical field. Our core competitive products include lentiviruses, adenoviruses, and adeno-associated viruses.
