01
Journal: Cell
IF: 64.5
Title: Local hyperthermia therapy induces browning of white fat and treats obesity
Method: Differentiated immortalized beige adipocytes were transfected with siNC or siRNA against Hnrnpa2b1 or adenovirus mediated Hnrnpa2b1 overexpression (Hanbio biotechnology, Shanghai, China) for two days. Cells were treated with 5mg/ml actinomycin D (Sigma-Aldrich, SBR00013) and harvested at indicated time points. The total RNA was isolated and analyzed by RT-qPCR. The degradation rate of mRNA Kd wasestimated by: lnðCt =C0Þ = Kdt,wheretisthetime asactinomycin D treatment for transcription inhibition. C is the mRNA concentration. The mRNA half-life t1/2 is calculated by: t1=2 = ln 2=Kd
Fig. 1
02
Journal: Cell
IF: 64.5
Title: LECT2, a Ligand for Tie1, Plays a Crucial Role in Liver Fibrogenesis
Method: The custom-made adenoviral vector carrying shRNA for mouse Tie1 (Ad-Tie1-shRNA), shRNA for mouse CD209a (Ad-CD209ashRNA), shRNA for mouse VEGFR2 (Ad-VEGFR2-shRNA), and mouse nonsense control shRNA (Ad-Ctrl shRNA) were purchased from Hanbio Biotechnology Co. Ltd. (Shanghai, China). The adenovirus were injected into mice (1 *10^8 pfu, via tail vein) to knock down Tie1, CD209a and VEGFR2, respectively.
Fig. 2
03
Journal: Cancer Cell
IF: 50.3
Title: TRIB3 Promotes APL Progression through Stabilization of the Oncoprotein PML-RARa and Inhibition of p53-Mediated Senescence
Method: HEK293T cells were transfected with PML-RARa plasmid. Cells were infected 12 hr later with TRIB3-orGFP-adenovirus and incubated with cycloheximide (CHX) (10 mg/mL) for indicated times.
Fig. 3
04
Journal: Cell Discovery
IF: 33.5
Title: Lineage-specific regulatory changes in hypertrophic cardiomyopathy unraveled by single-nucleus RNA-seq and spatial transcriptomics
Method: Human cardiac fibroblasts (passage 5-6, 8×104/well) were seeded before the day of transfection in 6-well or 24well plates. Cells (30%–50% confluent) were infected with adenovirus-vector-GFP or adenovirus-AEBP1-GFP (HanBio, HH20220630GX-AD02) in half volume medium at a multiplicity of infection (MOI) 400 or 300, respectively at 37°C for 4h. Then, the medium was changed to a full volume fresh culture medium. Eight hours later, cells were starved in 0.5% FBS for 12h. Subsequently, cells were treated with TGFβ (6ng/L, 100-21C-50UG, Peprotech) or vehicle for 48h. Then protein was extracted and the protein levels were analyzed by western blot assay.
Fig. 4
05
Journal: Nature Biomedical Engineering
IF: 28.1
Title: Local hyperthermia therapy induces browning of white fat and treats obesity
Method: As the most commonly employed viral vector for gene therapy and
vaccine development, recombinant adenovirus type-5 (Ad5) was used as a model virus.
Fig. 5
06
Journal: Nature Neuroscience
IF: 625
Title: Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage
Method: Primary neurons were infected with adenovirus expressing 3× flag-tagged PKM1
or PKM2 (Hanbio Biotechnology) at a multiplicity of infection of 50 and collected 48h after infection.
Fig. 7 Extended Data
