Long non-coding RNA（lncRNA）is a class of long non-coding RNA molecule with a length of more than 200nt, which is a by-product of RNA polymerase II transcription. It can regulate gene expression at various levels (epigenetic regulation, transcriptional regulation and post-transcriptional regulation, etc.). It is a research hotspot of genetics.
To verify the expression level and intracellular sublocalization of lncRNA;
Predict the miRNAs that lncRNAs may bind to through network database;
Informatic screening of lncRNA-bound miRNAs and further screening and validation by luciferase assay;
miRNA target gene prediction and functional enrichment analysis;
Screening, verification and related functional studies of miRNA target genes;
Verification of lncRNA-miRNA-mRNA regulatory network.
Professional software was used to predict the binding sites of miRNA, lncRNA and target genes;
psiCHECK plasmid unique to Promega Company was adopted;
The Luciferase dual fluorescence reporting system can obtain a wide linear quantitative range and extremely high sensitivity;
The instantaneous transfection technique is simple, rapid and short period, and is suitable for most cell types.
lncRNA overexpression and interference plasmid construction;
lncRNA overexpression and interference virus packaging;
Stable cell lines construction service based on lentivirus technology;
Verification service of lncRNA and target gene competitively bind with microRNA ;
Service of ceRNA mechanism research.
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