01
Journal: Cell
IF: 64.5
Title: Local hyperthermia therapy induces browning of white fat and treats obesity
Method: Adeno-associated virus (AAV)vector-mediated overexpression of mouse Hsf1 active form, Hnrnpa2b1, control (GFP), shRNA targeting Hnrnpa2b1 and scrambled control (GFP) were constructed, amplified, and purified by Hanbio biotechnology(Shanghai,China). A total of 50 ml of 1*10^9Vg/ml of each AAV diluted in PBS was injected into the inguinal fat pads of mice(Jimenezetal.,2018). Mice were monitored for changes in body weight, fat mass, cold tolerance, and oxygen consumption, then mice were sacrificed and tissues were dissected for further analysis.
Fig. 1
02
Journal: Cell
IF: 64.5
Title: Moderate UV Exposure Enhances Learning and Memory by Promoting a Novel Glutamate Biosynthetic Pathway in the Brain
Method: Intra-HPC injection of AAV-shRNA (0.5 mL/side, 1.3*10^12 vg/mL) against urocanase or random control shRNA (shRandom, 0.5 mL/side, 1.6*10^12 vg/mL)
Fig. 2
03
Journal: Science
IF: 56.9
Title: Rhythmic Cilia Changes Support SCN Neuron Coherence in Circadian Clock
Method: We generated Shh conditional knockout mice by bilateral injection of adeno-associated virus (AAV)–expressing Cre recombinase to the SCN of Shhfl/fl mice.
Fig. 3
04
Journal: Science
IF: 56.9
Title: Pituitary hormone α-MSH promotes tumor-induced myelopoiesis and immunosuppression
Method: To perform stereotaxic viral injections, mice were anesthetized with a pentobarbital (50 mg/kg, i.p.) and fixed into a stereotactic head frame. A heating pad was used to maintain the core body temperature of the animals at 36-37 °C. After exposing the skull via small incision, a small hole was drilled for injection. A volume of 500 nl viruses (HBAAV2/9 Hspa9 shPOMC-GFP, 1.2 × 10^12 viral genome (vg) /ml, Hanbio Biotech Co.) or control (HBAAV2/9-GFP, 1.6 × 10^12 vg /ml, Hanbio Biotech Co.) was injected using calibrated glass microelectrodes connected to an infusion pump (micro 4, WPI) at a rate of 100 nl/min.
Viral delivery was targeted to the pituitary gland (500 nl/side, coordinates, bregma: anteroposterior (AP): -2.5 mm; mediolateral (ML): ± 0.15 to 0.25 mm; dorsoventral (DV): -6.35 to 6.45 mm) (54). The skin incision was then closed with 4-0 silk thread. The shRNA sequence used was CTGCTTCAGACCTCCATAGAT. Experiments were conducted 3-4 weeks after injection.
Fig. 4
05
Journal: Cell Research
IF: 44.1
Title: METTL3-mediated N6-methyladenosine mRNA modification enhances long-term memory consolidation
Method: The wildtype Mettl3 and mutated Mettl3 were subcloned into pAAV2/DJ-CMV-MSC-RFP vector (HANBIO). The pAAV-RC and pHelper were co-transfected with pAAV2/DJ-CMV-wildtype-Mettl3-RFP (AAV2/DJ-WT-Mettl3), pAAV2/ DJ-CMV-mutated-Mettl3-RFP (AAV2/DJ-Mut-Mettl3) or pAAV2/DJ-CMV-MSC-RFP (AAV2/DJ-RFP) into AAV-293 cells by using LipoFiter transfection reagent (HANBIO) to generate the adeno-associated virus (AAV). Propagated AAV2/DJ in the AAV-293 cells were purified and the titer of virus was measured by plaque assays. The stock solutions of AAV2/DJ-WT-Mettl3, AAV2/DJ-Mut-Mettl3 and AAV2/DJ-RFP were 1.0–1.2×10^12 plaque formation unit (PFU)/ml, respectively.
Fig. 5
06
Journal: Signal Transduction and Targeted Therapy
IF: 39.3
Title: Eosinophils promote pulmonary matrix destruction and emphysema via Cathepsin L
Method: Mice were anesthetized using tribromoethanol 14 days prior to the establishment of the emphysema model and intratracheal administration of AAV2/5-m-Ctsl-EGFP (1.5 × 10^12 vg/mL) in 60 μLPBS to reduce CTSL expression in the lungs. The adeno-associated virus AAV2/5-EGFP NC (1.5×1012 vg/mL) was employed as a negative control. The effectiveness of the fusion protein was assessed through Western blotting.
Fig. 6
