When mitochondria are damaged and depolarized, under external stimuli such as ROS, nutrient deficiency, and cellular aging, the membrane potential of mitochondria dissipates. This is a prerequisite for the occurrence of mitophagy.
Mitochondria are encapsulated by autophagosomes to form mitophagosomes.
Mitophagosomes fuse with lysosomes.
The mitochondrial contents are degraded by lysosomes, and then these contents are recycled.
The probe relies on the mitochondrial membrane potential to accumulate on mitochondria.
The probe is pH-sensitive. In normal mitochondria (weak fluorescence), when mitophagy occurs and the pH drops, strong red fluorescence is emitted.
The mitophagy detection kit includes a lysosome probe that can co-localize with the probe for dual validation.
Mitophagy is the process by which cells selectively eliminate mitochondria through the autophagy mechanism. The selective removal of damaged or dysfunctional mitochondria is crucial for the functional integrity of the entire mitochondrial network and cell survival.
Research on mitophagy is a current hotspot in biology, and abnormalities in mitophagy are closely related to many diseases. Therefore, mitophagy detection kits hold significant biological importance in studying the specific molecular mechanisms and physiological significance of mitophagy.
Hanbio has independently developed mitochondria-specific fluorescent probes for precise localization and labeling of mitochondria. When used in combination with Hanbio's unique dual-fluorescent LC3 cell autophagy adenovirus, the mitophagy detection kit can accurately track the dynamic process of mitophagy in real time.
