If the virus is used for a short period of time after receiving the virus solution, it can be stored at 4 ℃ and preferably used up within a week；If long-term storage is required, it can be packaged and placed at -80 ℃ as needed to avoid repeated freeze-thaw during use；If stored in a liquid nitrogen tank, a dedicated storage tube needs to be replaced。In addition, if the virus has been stored for more than 6 months, Hanbio recommends retesting the virus titer before use.
AAV is an efficient gene delivery tool in vivo, which is suitable for animal experiments. However, because of its capacity and expression characteristics, AAV is not suitable for experiments with large genes or rapid expression after infection.
MOI, multiplicity of infection is a term used in virology and microbiology to describe the ratio of infectious agents, such as viruses or bacteria, to target cells in a culture or infection system.
The MOI is calculated by dividing the number of infectious agents (e.g., viral particles) by the number of target cells. For example, an MOI of 1 indicates that there is, on average, 1 infectious agent for every target cell in the culture.
Related calculation formula:
The amount of virus added per hole(μL)=MOI ×cells / virus titer(TU/mL or PFU/mL)×1000
MOI=(virus titer × Virus volume)/ cells
AAV is a single-stranded DNA virus, which needs to form a double-stranded appendage to infect cells for gene expression. At 1-2 weeks, the target sequence is also expressed, but at this time, it is in the stage of gradual accumulation of expression, and the expression level is low. Therefore, it is generally recommended to detect the expression of the target gene after 3-4 weeks.
Yes, tissue-specific infection can be achieved with adeno-associated virus (AAV). One of the key advantages of AAV vectors is their ability to target specific tissues or cell types, making them valuable tools for gene therapy and research applications.
Tissue-specific infection can be achieved by AAV site-specific injection and specific serotypes. Combined with specific promoters, it can be fined to the specific expression regulation of specific cells.
By selecting the appropriate AAV serotype, promoter, and delivery method, researchers can achieve tissue-specific transduction, allowing for targeted gene expression in specific tissues or cell types while minimizing off-target effects.
When diluting a virus for use in a laboratory setting, it's important to follow proper safety protocols and handling procedures. Here are general steps for diluting a virus:
Thawing: If the virus is stored frozen, remove it from the freezer and thaw it slowly at room temperature or in a refrigerator. Avoid rapid thawing, as it can damage the virus particles.
Diluent: Prepare an appropriate diluent for the virus. This could be a buffer solution such as phosphate-buffered saline (PBS) or a suitable cell culture medium. Ensure that the diluent is compatible with the virus and the target cells.
Taking lentivirus as an example：To achieve a 1x10^7 TU/mL titer from a 1x10^8 TU/mL original titer, you would need to mix 10 μL of the original virus with 90 μL of diluent, resulting in a total volume of 100 μL.
