Hanheng Biotechnology (Shanghai) Co., Ltd.
Viral Vector Construction

Viral Vector Construction

Viral vectors are often used as gene delivery vectors or disease research tools because of their unique gene delivery characteristics and targeting specificity. Adeno-associated virus (AAV) vectors, Lentivirus vectors and Adenovirus vectors are the most commonly used viral vectors

The production of all viral tools starts with the construction of expression plasmids, and Hanbio can provide various viral plasmid construction services.


Viral Vector Construction Services

  • Construction of lentivirus expression vector

  • Construction of adenovirus expression vector

  • Construction of adeno-associated virus expression vector

  • Construction of other virus expression vectors

  • Virus vector construction process


Viral Vector Construction Service Process

Construction of Plasmid Vector

Lentivirus Vector List

NO.

Regulation

Vector Name

Prokaryotic Antibiotic

Eukaryotic Antibiotic

Fluorescent Label

Promoter

LV005

OE

pHBLV-CMV-MCS-EF1-mCherry-T2A-Puromycin

Ampicillin

Puromycin

mCherry

CMV

LV006

OE

pHBLV-CMV-MCS-3flag-EF1-mCherry-T2A-Puromycin

Ampicillin

Puromycin

mCherry

CMV

LV007

OE

pHBLV-CMV-MCS-EF1-Puromycin

Ampicillin

Puromycin

NONE

CMV

LV008

OE

pHBLV-CMV-MCS-3flag-EF1-Puromycin

Ampicillin

Puromycin

NONE

CMV

LV011

OE

pHBLV-CMV-MCS-3flag-EF1-ZsGreen-T2A-Puromycin

Ampicillin

Puromycin

ZsGreen

CMV

LV012

OE

pHBLV-CMV-MCS-EF1-ZsGreen-T2A-Puromycin

Ampicillin

Puromycin

ZsGreen

CMV

LV052

OE

pHBLV-CMV-MCS-EF1-ZsGreen

Ampicillin

NONE

ZsGreen

CMV

LV05

OE

pHBLV-CMV-MCS-3flag-EF1-ZsGreen

Ampicillin

NONE

ZsGreen

CMV

LV081

OE

pHBLV-EF1-MCS-CMV-Puromycin

Ampicillin

Puromycin

NONE

EF1


Adenovirus Vector List

NO.

Regulation

Vector Name

Prokaryotic Antibiotic

Eukaryotic Antibiotic

Fluorescent Label

Promoter

Adeasy014

OE

pAdEasy-EF1-MCS-CMV-EGFP

Kanamycin

not have

EGFP

EF1

Adeasy015

OE

pAdEasy-EF1-MCS-CMV-mCherry

Kanamycin

not have

mCherry

EF1

Adeasy018

OE

pAdEasy-CMV

Kanamycin

not have

not have

CMV

Adeasy022

OE

pAdEasy-CMV-3flag

Kanamycin

not have

not have

CMV

Adeasy023

OE

pAdEasy-EF1-MCS-3flag-CMV-EGFP

Kanamycin

not have

EGFP

EF1

Adeasy024

OE

pAdEasy-EF1-MCS-CMV-LUC

Kanamycin

not have

Luciferase

EF1

Adeasy027

OE

pAdEasy-EF1-MCS-CMV-eRFP

Kanamycin

not have

eRFP

EF1

Adeasy030

OE

pAdEasy-EF1-MCS-3flag-CMV-mCherry

Kanamycin

not have

mCherry

EF1

Adeasy032

OE

pAdeasy-EF1-CircRNA-CMV-EGFP

Kanamycin

not have

EGFP

EF1

Adeasy019

RNAi

pAdEasy-U6

Kanamycin

not have

not have

U6

Adeasy020

RNAi

pAdEasy-U6-CMV-EGFP

Kanamycin

not have

EGFP

U6

Adeasy021

RNAi

pAdEasy-U6-CMV-mCherry

Kanamycin

not have

mCherry

U6

Adeasy028

RNAi

pAdEasy-U6-CMV-eRFP

Kanamycin

not have

eRFP

U6

Adeasy026

Cas9/gRNA

pAdEasy-U6-CMV-Cas9-2A-eRFP

Kanamycin

not have

eRFP

U6

Adeasy043

Cas9/gRNA

pAdEasy-U6-scaffold-CMV-Cas9-T2A-ZsGreen

Kanamycin

not have

ZsGreen

U6


AAV Vectors List

NO.

Regulation

Vector Name

Prokaryotic Antibiotic

Fluorescent Label

Promoter

AAV001

OE

pHBAAV-CMV-MCS-3flag-T2A-ZsGreen

Ampicillin

ZsGreen

CMV

AAV003

OE

pHBAAV-CMV-MCS-3flag-EF1-ZsGreen

Ampicillin

ZsGreen

CMV

AAV004

OE

pHBAAV-CAG-MCS-T2A-ZsGreen

Ampicillin

ZsGreen

CAG

AAV006

OE

pHBAAV-CAG-MCS-T2A-mCherry

Ampicillin

mCherry

CAG

AAV026

OE

pHBAAV-CMV-MCS-T2A-mCherry

Ampicillin

mCherry

CMV

AAV071

OE

pHBAAV-CMV-MCS-3flag

Ampicillin

CMV

AAV123

OE

pHBAAV-CMV-MCS-P2A-Luc2

Ampicillin

Luciferase

CMV

AAV007

Overexpression of cre induced

pHBAAV-CAG-DIO-MCS-T2A-ZsGreen

Ampicillin

ZsGreen

CAG

AAV008

Overexpression of cre induced

pHBAAV-CAG-DIO-MCS-T2A-mCherry

Ampicillin

mCherry

CAG

AAV027

Overexpression of cre induced

pHBAAV-CMV-DIO-MCS

Ampicillin

CMV

AAV028

Overexpression of cre induced

pHBAAV-CMV-DIO-MCS-T2Am-ZsGreen

Ampicillin

ZsGreen

CMV

AAV030

Overexpression of cre induced

pHBAAV-CAG-DIO-MCS

Ampicillin

CAG

AAV020

RNAi

pHBAAV-U6-MCS-CMV-mCherry

Ampicillin

mCherry

U6

AAV021

RNAi

pHBAAV-U6-MCS-CMV-EGFP

Ampicillin

EGFP

U6

AAV036

RNAi

pHBAAV-U6-MCS-CMV-ZsGreen

Ampicillin

ZsGreen

U6

AAV074

RNAi

pHBAAV-U6-MCS-CMV-eRFP

Ampicillin

eRFP

U6

AAV121

RNAi

pHBAAV-U6-MCS

Ampicillin

U6

AAV122

RNAi

pHBAAV-U6-MCS-CMV-Luc

Ampicillin

Luciferase

U6

AAV023

Cas9/gRNA

pHBAAV-CMV-Sacas9-U6-gRNA

Ampicillin

U6


Viral Vector Construction Service Avantages

  • Professional technical research team

  • Rich experience in molecular cloning

  • Multiple viral vectors are available for various expression systems

  • Construct different target vectors according to customer's needs


Get In Touch With Hanbio
Get In Touch With Hanbio

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