Hanheng Biotechnology (Shanghai) Co., Ltd.
Pseudovirus

Pseudovirus

A pseudovirus is a laboratory-created viral particle that mimics the structure of a real virus but lacks the ability to replicate and cause infection. Pseudoviruses are formed by encapsulating specific nucleic acid fragments within a viral capsid protein. Currently, the pseudoviruses launched by Hanbio Biotechnology are mainly lentivirus, adenovirus and adeno-associated virus. The quality of products is strictly controlled to ensure the stability between batches. Pseudoviruses are mainly used as positive control standard and internal control standard in viral nucleic acid test kits.

Main Applications of Peudoviruses

  • Carrying target genes for intracellular or in vivo gene overexpression or down-regulation

  • Carrying signature nucleic acid fragments for use as positive control or internal standard in viral nucleic acid detection kits

  • Carrying pathogenic viral capsid peptide as gene vaccine



Requirements for Peudovirus Standard

  • Pseudovirus standard and the target virus to be detected have the same or similar physicochemical properties, such as the protein particles wrapped in nucleic acid material

  • Pseudovirus standard carries the same nucleic acid fragment of the probe as the target virus to be detected

  • The purity of the Pseudovirus standard is sufficiently high that exogenous contaminating nucleic acids need to be removed during production

Hanbio Pseudovirus Advantages

  • High detection accuracy: Hanbio  provides two types of pseudovirus vectors, lentivirus and adenovirus, which are very similar to clinical pathogenic RNA and DNA viruses in terms of physicochemical properties, as internal control standard or positive controls, which can greatly improve the accuracy of virus detection and reduce the number of false-positive and false-negative results.

  • Purification and pollution free: Hanbio has self-developed pseudovirus purification system, which can effectively remove the contamination of DNA or RNA in the process of pseudovirus production, and avoid the influence of nucleic acid carried by non-pseudovirus on the test results to the maximum extent.

  • Accurate Titer: Hanbio  has developed its own pseudovirus titer measurement system, which can rapidly, efficiently and accurately measure pseudovirus titer.

Pseudovirus Service Process


pseudovirus

Pseudovirus Products List (Partial)


Product Name

Virus Name

Virus Types

AD-HB

Human bocavirus

DNA Virus

LV-Rhino

Rhinovirus A

RNA Virus

LV-NVGI

Norovirus GI

RNA Virus

LV-NGII

Norovirus GII

RNA Virus

LV-EGFR

Homo sapiens epidermal growth factor receptor (EGFR)

RNA Virus

LV-HBV

Hepatitis B virus

RNA Virus

LV-HMV-RvA68

Human metapneumovirus and rhinovirus A68

RNA Virus

LV-hCOV(NL63 OC43)

Human coronavirus NL63 and Human coronavirus OC43

RNA Virus

LV-hCOV(229E HKU1)

Human coronavirus 229E and HKU1(Human coronavirus HKU1)

RNA Virus

LV-HRSVA-EVD68

(Human respiratory syncytial virus A,HRSVA)and D68(Enterovirus D68 ,EV)

RNA Virus

LV-IBV-IAV

(Influenza B virus,IBV )and(Influenza A virus,IAV)

RNA Virus

LV-IAV-RPP30

Influenza A virus)and(Homo sapiens ribonuclease P/MRP subunit p30 (RPP30))

RNA Virus

LV-IAV

Influenza A virus

RNA Virus

LV-MP

Mycoplasma pneumoniae,MP

RNA Virus

AD-JCPyV

JC polyomavirus,JCPyV

DNA Virus

LV-SARS-CoV-2-RPP30

(Severe acute respiratory syndrome coronavirus 2)and(Homo sapiens ribonuclease P/MRP subunit p30 (RPP30))

RNA Virus

AD-HHV(B&Y)

(Human betaherpesvirus 5 )and(Human gammaherpesvirus 4)

DNA Virus

LV-SARS-COV-2-orf1

evere acute respiratory syndrome coronavirus 2

RNA Virus

LV-RPP30

(Homo sapiens ribonuclease P/MRP subunit p30 (RPP30))

RNA Virus

LV-IBV

Influenza B virus

RNA Virus

LV-HPIV1&2

(Human parainfluenza 1 virus)and(Human parainfluenza virus 2,Human orthorubulavirus 2)

RNA Virus

LV-HPIV3&4a

(Human parainfluenza 3 virus)and(Human parainfluenza virus 4a)

RNA Virus

LV-SARS-COV-2-HARV

Severe acute respiratory syndrome coronavirus 2)and(Human respiratory syncytial virus A)

RNA Virus

LV-HRSVA

Human respiratory syncytial virus A

RNA Virus

LV-hCOVNL63-HRSVA

Human coronavirus NL63)and(Human respiratory syncytial virus A)

RNA Virus

LV-HPIV1

Human parainfluenza 1 virus

RNA Virus

LV-HPIV2

Human parainfluenza virus 2

RNA Virus

LV-HPIV3

Human parainfluenza virus 3

RNA Virus

LV-hRV4

Human rubulavirus 4

RNA Virus

LV-hCOV(HKU1)

Human coronavirus HKU1

RNA Virus

LV-hCOV(OC43)

Human coronavirus OC43

RNA Virus

LV-hCOV(229E)

Human coronavirus 229E

RNA Virus

LV-RVc-HMV

(Rhinovirus C)and(Human metapneumovirus)

RNA Virus

LV-HMV

Human metapneumovirus

RNA Virus

LV- MAstV1

Mamastrovirus 1

RNA Virus

LV-HRVA

Human rotavirus A

RNA Virus

LV-HRVB

Human rotavirus B

RNA Virus

LV-SVGI

Sapovirus GI


LV-SVGII

Sapovirus GII


LV-SVGIV

Sapovirus GIV


LV-SVGV

Sapovirus GV


LV-SENRRL2338

Saccharopolyspora erythraea NRRL 2338


LV-SpAP200

Streptococcus pneumoniae AP200


LV-IAV

Influenza A virus


LV-RPP330

(Homo sapiens ribonuclease P/MRP subunit p30 (RPP30))


LV-ROV

rotavirus


LV-JS2

(Human rotavirus A)JS2



Get In Touch With Hanbio
Get In Touch With Hanbio

Contact Hanbio and leave your requirements. We will reply as soon as possible.