In the biomedical field, the transfer of plasmid vectors into mammalian cells using conventional transfection is the most widely used method. Although a number of more advanced gene introduction vector systems have been developed in recent years (e.g., lentiviral vectors, adenoviral vectors, AAV vectors, and piggyBac, etc.), conventional plasmid gene expression vectors are still used in the majority of laboratories, mainly due to their technical simplicity and efficient transfection rates in various types of cells.
One of the foundational plasmid vectors, construction of pbr322, constructed in 1977, laid the groundwork for modern plasmid design and has been instrumental in the development of genetic manipulation techniques.
Plasmids are widely used genetic manipulation tools for functional studies of coding genes, microRNAs, lncRNAs, promoter activity, transcription factors and 3'UTR regulation.
Mammalian expression vector construction
Virus vector construction (lentivirus, adenovirus, AAV packaging service)
Gene reporter vector construction
Gene mutation
Vector modification
miRNA, lncRNA expression vector construction
HANBIO provide professional technical research team for plasmid vector construction service.
HANBIO has rich experience in molecular cloning.
Multiple tool vectors are available for various expression systems by HANBIO.
HANBIO can offer different target vector construction in biotechnology according to customer's needs.
Contact Hanbio and leave your requirements. We will reply as soon as possible.
