MicroRNA(miRNA) is a class of endogenous non-coding RNA found in eukaryotes with regulatory functions. They are about 20~25 nt. miRNAs are evolutionally-conserved and have a rich variety of species, they are not translated into proteins, but play an important role in regulating gene expression. miRNA can specifically bind to complementary regions within messenger RNA(mRNA) to promote its degradation or inhibit translation. miRNA is widely involved in cell proliferation, differentiation, apoptosis and cell cycle regulation, and plays a key role in gene experiments.
miRNA can be overexpressed using miRNA mimics or constructing pri-miRNA onto expression vectors. The downregulation of miRNA is constructed mainly by sponge or miRNA inhibitor (miRNA complementary single-chain). Sponge technology connects several consecutive reverse and complementary sequence of mature miRNA sequence to enable them to be expressed in cells and acted as a sponge for target microRNA to achieve the purpose of down-regulating miRNA.
Luciferase is a general term for enzymes that can produce bioluminescence in nature, which the most representative are two types of luciferase enzymes from firefly and renilla. Under the catalysis of luciferase, luciferin substrate can be efficiently converted into oxyluciferin and emit strong light at the same time. In view of this, the researchers used luciferase to develop an extremely sensitive and user-friendly gene reporting system. At present, this system is widely used for the validation of miRNA target gene, analysis of transcription factor binding sites and promoter activity, RNA degradation, signal transduction, drug screening, in vivo imaging and other fields.
miRNA overexpression and interference plasmid construction.
miRNA overexpression and interference virus packaging.
Stable cell lines construction services based on lentivirus technology.
Dual luciferase assay validation services for miRNA and target genes.
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