Circular RNAs (circRNAs) are a type of non-coding RNA molecules that lack a 5' cap and a 3' poly(A) tail and form a circular structure with covalent bonds. Distinct from traditional linear RNAs, circRNAs possess a closed circular structure and are abundantly present in the eukaryotic transcriptome. The majority of circRNAs are composed of exon sequences and exhibit conservation across different species, as well as tissue-specific and expression patterns of developmental stage-specific.
Hanbio has developed an efficient circular RNA (circRNA) overexpression vector system. These vectors can be transfected into mammalian cells to achieve high levels of circRNA expression and promote efficient circularization.
Recent studies indicated that circRNAs are enriched with miRNA binding sites, functioning as miRNA sponges in cells. By adsorbing miRNAs，circRNAs alleviate the inhibitory effect of miRNAs on their target genes, thereby elevating the expression level of the target genes. This mechanism is referred to as the competitive endogenous RNA (ceRNA) mechanism. Investigating the regulatory mechanism of circRNA requires identification of the miRNAs that interact with circRNAs, and dual-luciferase reporter gene assays can be used to prove the interaction between miRNAs and circRNAs.
The CMV promoter from human cytomegalovirus can efficiently express the target circRNA.
The upstream and downstream circularization elements, front circ-signal and back circ-signal, can efficiently promote the circularization of the expressed circRNA.
The linearized vector is very convenient for seamless cloning of the target circRNA, greatly reducing the chance of irrelevant sequences participating in circularization.
Construction of circRNA overexpression and interference plasmids.
Packaging of circRNA overexpression and interference virus.
Stable cell lines construction services based on lentivirus technology.
Dual-luciferase verification services for circRNA and target genes.
Precise circularization verification of circRNA.
ceRNA mechanism research services.
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