Cell proliferation is the basis of organism growth, development, reproduction and heredity, and is an important life characteristic of organism. Cells usually proliferate by dividing. Cytotoxic is a simple cell-killing event caused by cells or chemicals, which is not dependent on apoptosis or necrosis.
Cell Counting Kit-8, referred to as CCK-8 Kit, an alternative method of MTT assay, is a rapid and highly sensitive detection kit based on WST (water-soluble tetrazolium salt, chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfobenzene)-2H-tetrazolium monosodium salt) widely used in cell proliferation and cytotoxicity. It can be used for drug screening, cell proliferation assay, cytotoxicity assay, tumor drug sensitivity, and other tests.
Dry and keep in the dark at 4℃, valid for one year (recommended). -20℃ dry and stored in the dark, valid for two years.
| Code No. | Product Name | Usage Counter | Volume |
| HB-CCK-8-500 | CCK-8 cell proliferation detection kit | 500 tests | 5mL |
| HB-CCK-8-1000 | CCK-8 cell proliferation detection kit | 1000 tests | 10mL |
| HB-CCK-8-3000 | CCK-8 cell proliferation detection kit | 3000 tests | 30mL |
| HB-CCK-8-10000 | CCK-8 cell proliferation detection kit | 10000 tests | 100mL |
CCK-8 cell proliferation detection kit is a rapid sensitivity kit based on WST-8 (chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfonyl benzene)), which is widely used in cell proliferation and cytotoxicity detection. The WST-8 is an upgrade of MTT and works as follows: In the presence of electron-coupled reagents, WST-8 can be reduced by the mitochondrial dehydrogenase to produce the highly water-soluble orange-yellow formazan (Figure 1-2), the color of which is proportional to the proliferation of the number of surviving cells and inversely proportional to cytotoxicity. OD values measured at 450 nm wavelength by enzyme-labeled apparatus can indirectly reflect the number of living cells. The CCK-8 method is widely used in the molecular biology reagents list for applications such as drug screening, cell proliferation assays, cytotoxicity assays, tumor drug susceptibility assays, and biological factor activity detection.
| Comparison item | MTT assay | XTT assay | WST-1 assay | CCK-8 assay |
| Water solubility of formzan product | Poor (Need to add organic solvent to dissolve and then test) | Good | Good | Good |
| Shape of product | Powder | 2 bottles of solution | solution | 1 bottle of solution |
| Method of Use | The powder was prepared into a solution before use. | Preparation as needed. | Out-of-the-box. | Out-of-the-box |
| Sensitivity of detection | High | Very high | Very high | High |
| Detection time | Long | Short | Short | Shortest |
| Wavelength of detection | 500-600nm | 420-480nm | 420-480nm | 430-490nm |
| Cytotoxic | High, complete loss of cell morphology | Very low, cell morphology unchanged. | Very low, cell morphology unchanged. | Very low, cell morphology unchanged. |
| Stability of reagents | General | Poor | General | Very good |
| Batch sample testing | Yes | Very suitable | Very suitable | Very suitable |
| Convenience | General | Convenient | Convenient | Very convenient |
Note:
1. Phenol red and serum did not interfere with the detection of CCK-8 method.
2. The cytotoxicity of this kit is very low, so after adding WST-8 for color development, the best assay time can be found by repeatedly reading with the microplate reader at different times.
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