Adeno-Associated Virus (AAV) belongs to the family of parvovirus and is a non-enveloped single-stranded DNA virus, with a genome size of 4.7 kb composed of two open reading frames (ORFs) flanked by inverted terminal repeat sequences (ITRs) of 145 nucleotides each. AAV vectors are one of the most commonly used vectors for a variety of gene therapy applications and have been widely used in clinics and basic research mainly owing to their capable of transducing a wide range of species and tissues in vivo with no evidence of toxicity, and it generates relatively mild innate and adaptive immune responses as well as their long-term transgene expression and steady physical properties.
Services | Products | Titer | Application |
| Conventional promoter/serotype AAV | 1012v.g/ml or 1013v.g/ml | Suitable for most tissue infections |
Specific promoter/serotype AAV | 1012v.g/ml or 1013v.g/ml | Suitable for specific target organ/tissue infections, with strong specificity |
NO. | Regulation | Vector Name | Prokaryotic Antibiotic | Fluorescent Label | Promoter |
AAV001 | OE | pHBAAV-CMV-MCS-3flag-T2A-ZsGreen | Ampicillin | ZsGreen | CMV |
AAV003 | OE | pHBAAV-CMV-MCS-3flag-EF1-ZsGreen | Ampicillin | ZsGreen | CMV |
AAV004 | OE | pHBAAV-CAG-MCS-T2A-ZsGreen | Ampicillin | ZsGreen | CAG |
AAV006 | OE | pHBAAV-CAG-MCS-T2A-mCherry | Ampicillin | mCherry | CAG |
AAV026 | OE | pHBAAV-CMV-MCS-T2A-mCherry | Ampicillin | mCherry | CMV |
AAV071 | OE | pHBAAV-CMV-MCS-3flag | Ampicillin | / | CMV |
AAV123 | OE | pHBAAV-CMV-MCS-P2A-Luc2 | Ampicillin | Luciferase | CMV |
AAV007 | Overexpression of cre induced | pHBAAV-CAG-DIO-MCS-T2A-ZsGreen | Ampicillin | ZsGreen | CAG |
AAV008 | Overexpression of cre induced | pHBAAV-CAG-DIO-MCS-T2A-mCherry | Ampicillin | mCherry | CAG |
AAV027 | Overexpression of cre induced | pHBAAV-CMV-DIO-MCS | Ampicillin | / | CMV |
AAV028 | Overexpression of cre induced | pHBAAV-CMV-DIO-MCS-T2Am-ZsGreen | Ampicillin | ZsGreen | CMV |
AAV030 | Overexpression of cre induced | pHBAAV-CAG-DIO-MCS | Ampicillin | / | CAG |
AAV020 | RNAi | pHBAAV-U6-MCS-CMV-mCherry | Ampicillin | mCherry | U6 |
AAV021 | RNAi | pHBAAV-U6-MCS-CMV-EGFP | Ampicillin | EGFP | U6 |
AAV036 | RNAi | pHBAAV-U6-MCS-CMV-ZsGreen | Ampicillin | ZsGreen | U6 |
AAV074 | RNAi | pHBAAV-U6-MCS-CMV-eRFP | Ampicillin | eRFP | U6 |
AAV121 | RNAi | pHBAAV-U6-MCS | Ampicillin | / | U6 |
AAV122 | RNAi | pHBAAV-U6-MCS-CMV-Luc | Ampicillin | Luciferase | U6 |
AAV023 | Cas9/gRNA | pHBAAV-CMV-Sacas9-U6-gRNA | Ampicillin | / | U6 |
Tissues | Serotypes |
Heart | AAV9 |
Liver | AAV8/9 |
Whole Brain (traverse the blood brain barrier) | AAV-BBB |
Brain(partial delivery) | AAV9/AAV2/AAV5 |
Retrograde delivery | AAV-Retro |
Spinal Cord | AAV5/AAV1/AAV9 |
Adipose Tissue | AAV9 |
Skeletal Muscle | AAV9 |
Eyes | AAV2/AAV-DJ |
Lung | AAV6 |
Kidney | AAV2/AAV9 |
Intestine | AAV1/AAV5 |
Blood Vessels (mainly endothelium) | AAV1/AAV5 |
Pancrea | AAV8 |
Tissues | Promoter | Characteristics |
Neurons | hSyn | Neuron-specific promoter |
mecp2 | Shorter neuron-specific promoter | |
C-fos | Excitatory neuron promoter | |
CaMKIIα | forebrain excitatory neurons | |
Nms | Neural precursor cell-specific promoter | |
TUBA1A(Tα1) | Early neuron-specific promoter | |
hVGAT | GABAergic neuron/interneuron-specific promoter | |
TH | Dopaminergic neuron-specific promoter | |
gfaABC1D (minimal GFAP) | Astrocyte-specific promoter | |
CD68 | Activated microglia-specific promoter | |
ibal | Activated and resting microglia-specific promoter | |
TMEM119 | Microglia-specific promoter | |
CNP(NPPC) | Oligodendrocyte or Schwann cell-specific promoter | |
MBP | Oligodendrocyte-specific promoter | |
PLP | Oligodendrocyte-specific promoter | |
pdgfra | Oligodendrocyte precursor cell-specific promoter | |
NG2 | Oligodendrocyte precursor cell-specific promoter | |
Pirt | Sensory neuron-specific promoter | |
ALDH1L1 | L1 Hypothalamic astrocyte-specific promoter | |
Heart | cTNT | Cardiomyocyte-specific promoter |
POSTN | Cardiac fibroblast-specific promoter | |
TCF21 | Cardiac fibroblast-specific promoter | |
Muscle | MHCK7 | Muscle-specific promoter |
SM22a | Smooth muscle-specific promoter | |
MHCK7 | Muscle-specific promoter | |
ACTA1 (synthesized Spc5-12) | Muscle cell-specific promoter | |
MYOG | Cardiac fibroblast-specific promoter | |
Lung | SP-C (SFTPC) | Lung epithelial cell-specific promoter |
Liver | TBG | Liver-specific promoter |
Kidney | nphs1 | Kidney podocyte-specific promoter |
nphs2 | Kidney podocyte-specific promoter | |
Ksp-cadherin | Renal tubular epithelial cell-specific promoter | |
Pancrea | padx1（pdx1） | Pancreas-specific promoter |
Insulin2 | Pancreas-specific promoter | |
Adipose Tissue | FABP4 (aP2) | Adipocyte-specific promoter |
adiponectin | Adipocyte-specific promoter | |
UCP1 | Brown adipocyte-specific promoter | |
Myf5 | Adipocyte precursor cell-specific promoter | |
Endothelium | TIE | Endothelial cell-specific promoter |
Retina | rpe65 | Retina-specific promoter |
Arr3 | Retinal cone cell-specific promoter | |
hrk | Retinal cone and rod cell-specific promoter | |
Prostate | DD3 | Prostate cancer cell-specific promoter |
Skin | Keratin14（K14） | Skin epidermal cell-specific promoter |
K14 | Keratin promoter | |
Macrophages | F4/80 | Macrophage-specific promoter |
Bone | COL2A1 | Chondrocyte-specific promoter |
Stem Cells | Nanog | Pluripotent stem cell promoter |
Serotype Name | Specific Cells |
AAV-shH10 | Retinal Müller cells |
AAV-Vec | Endothelial cells |
AAV-MG | Microglia |
AAV-MG1.2 | Microglia |
AAV-br1 | Brain vascular endothelial cells |
AAV-BI30 | Brain vascular endothelial cells |
AAV-eVEC | Peripheral vascular endothelial cells |
AAV-anc80L | Retina/Inner ear |
AAV-ie | Inner ear |
AAV-BBB | traverse the blood brain barrier |
AAV-GL | Retina |
AAV-NN | Retina |
AAV-capB10 | Brain |
AAV-BLS | Bladder |
Safety: AAV (Adeno-Associated Virus) vectors have shown non-pathogenicity in humans and been used to deliver genetic material into target cells to cure or treat disease.
Long-term transgene expression: AAV (Adeno-Associated Virus) vectors have a low immunogenic profile, which means they are less likely to trigger an immune response in the host. Furthermore, AAV vectors are known for their ability to provide long-term transgene expression. Studies have shown that transgene expression delivered by AAV vectors can persist for at least 6 months and even longer in some cases.
A wide range of species and tissues in vivo: AAV (Adeno-Associated Virus) is known for its high titer, with levels reaching over 10^13 viral genomes per milliliter. This high titer allows for efficient gene delivery and expression in target tissues. AAV is also capable of transducing a wide range of species and tissues in vivo with no evidence of toxicity.
Low cytotoxicity: Strict purification process
Qualified: Strictly follow quality control standards
High titer: Reaching up to 10^13 viral genomes per milliliter (vg/mL)
Selection: Wide range of serotypes, capable of infecting almost all tissues and organs; a variety of tissue-specific promoters
Testing Items | Testing Method | Result |
Mycoplasma | PCR | Negative |
Endotoxin | LAL | Qualified |
Bacteria | Culture | Negative |
Fungi | Culture | Negative |
Active Titer | Infection | ≥10^12 v.g/ml |
Moderate UV Exposure Enhances Learning and Memory by Promoting a Novel Glutamate Biosynthetic Pathway in the Brain
（CELL(IF=41.582, 2018), University of Science and Technology of China）
LECT2, a Ligand for Tie1, Plays a Crucial Role in Liver Fibrogenesis
（CELL(IF=41.582, 2019), Southern Medical University）
Local hyperthermia therapy induces browning of white fat and treats obesity
（CELL(IF=64.5, 2022)，East China Normal University）
Neuraminidase 1 is a driver of experimental cardiac hypertrophy
（European Heart Journal(IF=29.983, 2021)， China Pharmaceutical University）
Targeting E3 Ubiquitin-Ligase WWP1 Prevents Cardiac Hypertrophy Through Destabilizing DVL2 via Inhibition of K27-Linked Ubiquitination
（CIRCULATION(IF=29.690, 2021)，China Astronaut Research and Training Center）
N6-methyladenosine-modified TRAF1 promotes sunitinib resistance by regulating apoptosis and angiogenesis in a METTL14-dependent manner in renal cell carcinoma
（Molecular Cancer(IF=27.401, 2022)，Sir Run Run Shaw Hospital, Zhejiang University School of Medicine）
Sja-miR-71a in Schistosome egg-derived extracellular vesicles suppresses liver fibrosis caused by schistosomiasis via targeting semaphorin 4D
（Journal of Extracellular Vesicles(IF=25.841, 2020)，Sun Yat-sen University）
Transcriptional switch of hepatocytes initiates macrophage recruitment and T cell suppression in endotoxemia
（Journal of Hepatology(IF=25.083, 2022)，The Third Xiangya Hospital, Central South University）
TRIB3 Interacts With β-Catenin and TCF4 to Increase Stem Cell Features of Colorectal Cancer Stem Cells and Tumorigenesis
（Gastroenterology(IF=22.682, 2019)，Chinese Academy of Medical Sciences and Peking Union Medical College）
Reducing Hypothalamic Stem Cell Senescence Protects against Aging-Associated Physiological Decline
（Cell Metabolism(IF=21.567, 2020)，Xiangya Hospital of Central South University）
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